Transgenic Assay:Article Title: Immortalized mesenchymal stromal cells overexpressing alpha-1 antitrypsin protect acinar cells from apoptotic and ferroptotic cell death.
Article Snippet: Antibody Specificity Technique Source Dilution Rabbit Anti- SV40 Large T Antigen Transgenic levels of total SV40 Large T Antigen protein IC, WB Cell Signaling Technology IC- 1:100, WB- 1:1000 PE Mouse Anti- human CD29 Human integrin β1 (ITGB1) FC BioLegend 1:100 PE Mouse Anti- Human CD44 Homing cellular adhesion molecule (HCAM) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD90 Thymocyte differentiation antigen 1 (Thy- 1) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD45 Leukocyte common antigen (LCA) FC BD Pharmingen 1:100 Rabbit Anti- BAX N- terminus (N- 20) of Bax WB Santa Cruz Biotechnology 1:1000 Rabbit Anti- Cleaved Caspase- 3 Asp175 of Caspase- 3 WB Cell Signaling Technology 1:1000 Rabbit Anti- Cleaved PARP Asp214 of PARP WB Cell Signaling Technology 1:1000 Mouse Anti- p53 Ser20 of p53 WB Cell Signaling Technology 1:1000 Rabbit Anti- p38 p38α, −β or - γ MAPK protein WB Cell Signaling Technology 1:1000 Rabbit Anti- JNK/SAPK JNK1, JNK2 or JNK3 protein WB Cell Signaling Technology 1:1000 Rabbit Anti- Occludin Polyclonal - WB Thermo Fisher Scientific 3 μg/mL Rabbit Anti- ZO- 1 Polyclonal - WB Proteintech 1:5000 Rabbit Anti- FTH1 Amino- terminal region of FTH1 WB Cell Signaling Technology 1:1000 Rabbit Anti- PDI C81H6 of PDI WB Cell Signaling Technology 1:1000 Rabbit Anti- GPX4 Polyclonal - IC Thermo Fisher Scientific 1:50 Rabbit Anti- β- Actin Cytoplasmic Actin isoforms, β- Actin and cytoplasmic γ- Actin WB Cell Signaling Technology 1:1000 Goat Anti- Rabbit IgG, HRP- linked Antibody Rabbit polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Horse Anti- Mouse IgG, HRP- linked Antibody Mouse polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Goat anti- Rabbit IgG Cross- Adsorbed Secondary Antibody, Alexa FluorTM 488 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 Goat anti- Rabbit IgG Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 568 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 15824934, 2024, 20, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70093 by E gyptian N ational Sti.
Western Blot:Article Title: Immortalized mesenchymal stromal cells overexpressing alpha-1 antitrypsin protect acinar cells from apoptotic and ferroptotic cell death.
Article Snippet: Antibody Specificity Technique Source Dilution Rabbit Anti- SV40 Large T Antigen Transgenic levels of total SV40 Large T Antigen protein IC, WB Cell Signaling Technology IC- 1:100, WB- 1:1000 PE Mouse Anti- human CD29 Human integrin β1 (ITGB1) FC BioLegend 1:100 PE Mouse Anti- Human CD44 Homing cellular adhesion molecule (HCAM) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD90 Thymocyte differentiation antigen 1 (Thy- 1) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD45 Leukocyte common antigen (LCA) FC BD Pharmingen 1:100 Rabbit Anti- BAX N- terminus (N- 20) of Bax WB Santa Cruz Biotechnology 1:1000 Rabbit Anti- Cleaved Caspase- 3 Asp175 of Caspase- 3 WB Cell Signaling Technology 1:1000 Rabbit Anti- Cleaved PARP Asp214 of PARP WB Cell Signaling Technology 1:1000 Mouse Anti- p53 Ser20 of p53 WB Cell Signaling Technology 1:1000 Rabbit Anti- p38 p38α, −β or - γ MAPK protein WB Cell Signaling Technology 1:1000 Rabbit Anti- JNK/SAPK JNK1, JNK2 or JNK3 protein WB Cell Signaling Technology 1:1000 Rabbit Anti- Occludin Polyclonal - WB Thermo Fisher Scientific 3 μg/mL Rabbit Anti- ZO- 1 Polyclonal - WB Proteintech 1:5000 Rabbit Anti- FTH1 Amino- terminal region of FTH1 WB Cell Signaling Technology 1:1000 Rabbit Anti- PDI C81H6 of PDI WB Cell Signaling Technology 1:1000 Rabbit Anti- GPX4 Polyclonal - IC Thermo Fisher Scientific 1:50 Rabbit Anti- β- Actin Cytoplasmic Actin isoforms, β- Actin and cytoplasmic γ- Actin WB Cell Signaling Technology 1:1000 Goat Anti- Rabbit IgG, HRP- linked Antibody Rabbit polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Horse Anti- Mouse IgG, HRP- linked Antibody Mouse polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Goat anti- Rabbit IgG Cross- Adsorbed Secondary Antibody, Alexa FluorTM 488 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 Goat anti- Rabbit IgG Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 568 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 15824934, 2024, 20, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70093 by E gyptian N ational Sti.
Bioprocessing:Article Title: Immortalized mesenchymal stromal cells overexpressing alpha-1 antitrypsin protect acinar cells from apoptotic and ferroptotic cell death.
Article Snippet: Antibody Specificity Technique Source Dilution Rabbit Anti- SV40 Large T Antigen Transgenic levels of total SV40 Large T Antigen protein IC, WB Cell Signaling Technology IC- 1:100, WB- 1:1000 PE Mouse Anti- human CD29 Human integrin β1 (ITGB1) FC BioLegend 1:100 PE Mouse Anti- Human CD44 Homing cellular adhesion molecule (HCAM) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD90 Thymocyte differentiation antigen 1 (Thy- 1) FC BD Pharmingen 1:100 PE Mouse Anti- Human CD45 Leukocyte common antigen (LCA) FC BD Pharmingen 1:100 Rabbit Anti- BAX N- terminus (N- 20) of Bax WB Santa Cruz Biotechnology 1:1000 Rabbit Anti- Cleaved Caspase- 3 Asp175 of Caspase- 3 WB Cell Signaling Technology 1:1000 Rabbit Anti- Cleaved PARP Asp214 of PARP WB Cell Signaling Technology 1:1000 Mouse Anti- p53 Ser20 of p53 WB Cell Signaling Technology 1:1000 Rabbit Anti- p38 p38α, −β or - γ MAPK protein WB Cell Signaling Technology 1:1000 Rabbit Anti- JNK/SAPK JNK1, JNK2 or JNK3 protein WB Cell Signaling Technology 1:1000 Rabbit Anti- Occludin Polyclonal - WB Thermo Fisher Scientific 3 μg/mL Rabbit Anti- ZO- 1 Polyclonal - WB Proteintech 1:5000 Rabbit Anti- FTH1 Amino- terminal region of FTH1 WB Cell Signaling Technology 1:1000 Rabbit Anti- PDI C81H6 of PDI WB Cell Signaling Technology 1:1000 Rabbit Anti- GPX4 Polyclonal - IC Thermo Fisher Scientific 1:50 Rabbit Anti- β- Actin Cytoplasmic Actin isoforms, β- Actin and cytoplasmic γ- Actin WB Cell Signaling Technology 1:1000 Goat Anti- Rabbit IgG, HRP- linked Antibody Rabbit polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Horse Anti- Mouse IgG, HRP- linked Antibody Mouse polyclonal and monoclonal antibodies WB Cell Signaling Technology 1:1000 Goat anti- Rabbit IgG Cross- Adsorbed Secondary Antibody, Alexa FluorTM 488 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 Goat anti- Rabbit IgG Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM 568 Rabbit polyclonal and monoclonal antibodies IC Thermo Fisher Scientific 1:500 15824934, 2024, 20, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70093 by E gyptian N ational Sti.
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